Using chemical "nanoblasts" that punch tiny holes in the protective membranes of cells, Georgia Tech researchers have demonstrated a new technique for getting therapeutic small molecules, proteins, and DNA directly into living cells. The work is believed to be the first to use activation of reactive carbon nanoparticles by lasers for medical applications.
The technique begins with introducing particles of carbon black measuring 25 nm in diameter into the fluid surrounding the cells into which the therapeutic agents are to be introduced. Bursts of near-infrared light from a femotosecond laser are then applied to the fluid at a rate of 90 million pulses per second. The carbon nanoparticles absorb the light, which makes them hot. The hot particles then heat the surrounding fluid to make steam. The steam reacts with the carbon nanoparticles to form hydrogen and carbon monoxide.
According to the researchers, one of the most significant uses for this technology could be for gene-based therapies, which offer great promise in medicine, but whose progress has been limited by the difficulty of getting DNA and RNA into cells.
