Researchers from Purdue University (West Lafayette, IN) are developing an integrated biosensing platform aimed at detecting and monitoring mosquito-borne diseases faster and cheaper than current methods, to aid in preventing virus outbreaks and their devastating effects.

Lia A Stanciu, a Purdue professor of materials engineering, is leading the research and development of the technology. Additional researchers are Ernesto Marinero, professor of materials engineering and electrical and computing engineering; and Richard Kuhn, professor and department head of biological sciences and director of the Purdue Institute for Inflammation, Immunology and Infectious Diseases. Kuhn also led the research team that were the first to determine the structure of the Zika virus. Stanciu says that current detection methods for neglected tropical diseases are often time-consuming, expensive and complicated.

The researchers have developed an amperometric biosensor that utilizes functionalized nanoparticles that specifically bind to the target viruses’ DNA or RNA. When the binding occurs, there is a change in the device resistance, which the sensor employs to unambiguously detect the presence of the virus. The sensor can then determine whether or not a blood or mosquito sample has the virus and how much of the virus is present. The sensor relies on an agent that will only respond to the intended virus to be detected.

“We’ve used mosquito samples on our laboratory scale sensor and we’ve been able to detect the virus showing a high sensitivity rate to low concentrations of the virus,” Stanciu says. “We’ve been especially interested in the dengue and Zika virus because it’s the same mosquito that transmits both diseases, so our technology would be able to quickly detect one of those diseases using the same platform.”

“Our first and foremost goal is to have a point of care, potentially a personal device, which is simple to use. This would allow people to detect the virus promptly without having to go to a hospital, which would have significant impacts in developing countries,” he says. “However, we’re also working on an autonomous device that is deployable in remote field areas that are difficult to access or difficult to perform in-site detection, to monitor outbreaks in these areas.”

The device will operate through a low-power wireless network and will use thin-film rechargeable batteries combined with thin-film photovoltaics to power and harvest energy from the environment to maintain functionality and performance without human intervention. When a disease is detected in a mosquito sample it will send an alert to health control officials about the potential threat.

Stanciu says they are seeking funding to further develop the technology.


Medical Design Briefs Magazine

This article first appeared in the May, 2017 issue of Medical Design Briefs Magazine.

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