A new microfluidic device can detect the SARS-CoV-2 virus with high accuracy and speed, using a unique DNA/RNA duplex technology. The device can prove to be a game-changer in the fight against the ongoing COVID-19 pandemic.
The device can distinguish SARS-CoV-2 from other respiratory viruses such as the Middle East respiratory syndrome coronavirus (MERS-CoV). The device uses a DNA biosensor to detect RNA from SARS-CoV-2 in human saliva. When viral RNA binds to the DNA biosensor, it forms a DNA/RNA duplex. This is then detected by the sensor, which can pick up as little as 10 attomolar of the virus RNA.
The device is based on microfluidics, a technology that controls the flow of very small amounts of liquids through tiny channels. With microfluidics, the new device controls the flow of saliva to allow the DNA biosensor to detect viral RNA. The DNA/RNA duplex is then detected with fluorescence measurements.
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